Standard RNA isolation and cDNA preparation protocols are used for Denome qPCR assay. RNA samples with low-integrity will negatively affect the qPCR assay.
We predict 8000 genes with 92% Pearson Correlation and an average of ±0.7 fold standard error.
We have tested our kit in various human and mouse tissues including colon, skin, breast, lung, fibroblast, macrophage, neuron, muscle, kidney, neuron and testis.
Denome Technology offers to predict expression of ~8000 genes, which enable to perform accurate GO analysis that each GO term is represented by at least 20 genes.
Standard RNA isolation and cDNA preparation protocols are used for Denome qPCR assay. RNA samples with low-integrity will negatively affect the qPCR assay.
Results will be analyzed and returned to user within 36 hours.
qPCR data will be processed by the Denome Tech machine learning algorithm. Expression values of genes will be delivered in excel file. Results will be plotted as volcano plots and heatmap together with GO analysis. Researchers can contact our bioinformatics department for additional analysis.
We strongly recommend testing the gene of interest by qPCR using gene-specific primers.
We strongly recommend our customers to use calibration DNA to test the efficiency of their instrument.
Please login to Denome Technologies web site and upload your raw qPCR data directly. We kindly ask customers to label their samples in their qPCR output file as the same sample name will be used in the volcano and heatmap plots.
We provide a unique accession number for each experiment. Researchers can generate password to give access to reviewers and provide full access after their study is published.