4C-seq analysıs
4C-Seq utilizes circularized chromosome conformation capture (4C) technique in coupled with high-throughput sequencing to identify genomic regions that interact and/or come into contact with genomic sites/loci of interest (viewpoint).
Case Study
Can we detect the region(s) that interact with the promoter region of our gene of interest and identify regulatory elements such as enhancers within them?
By performing 4C-Seq, we can visualize the interacting regions of a specific genomic site/loci of interest (Figure 1). Further analysis can then reveal if these interacting sites account for regulatory regions and could then identify interactions such as promoter-enhancer. This could give insight on the distal regulatory mechanisms of genes under varying conditions such as in disease.
Figure 1: Interactions between a specific genomic region/loci (viewpoint) and distal regions along the chromosome.
Standard Analysis Workflow:
- FREE initial consulation includes discussion on project objectives and result expectation, analysis method proposal, timeline and cost estimation.
- Standard data analysis includes primary sequencing reads quality control to downstream reads quatification and functional profiling for up to 12 samples. Please refer to analysis catalog for detail analysis workflow and result description.
- Publication ready figures/plots includes customize processing of analysis result plots such as spliting by samples group/conditions, color selection, highligting specific components within the plot etc. Only applies to analysis result of samples in package analysis.
- Publication submission consulation includes compilation of publication ready analysis result, scientific writing review, scientific plots review or customization, consultation for reviewer queries for up to 5 hours in total.
- Data integration includes intergration of existing pre-processed data for up to two applications between RNA-Seq, ChIP-Seq, or ATAC-Seq.
- TCGA differential expression and correlation analysis profiles various cancer types and identifies the most associated genes and pathways related to gene of interest. Please refer to our TCGA Analysis page for more information.
- Customize bioinformatic analysis: USD 370 / hour
- Standard data analysis: USD 110 / sample
- Bioinformatics consulation: USD 110 / hour
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We provide customized NGS analysis service to our customers. Our bioinformatics team generate publication quality data based on your requirements.
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