Denome DLBCL Subtyping qPCR Kit

The Denome DLBCL Subtyping qPCR Kit is designed to quantify the expression of a set of core genes (Denome DLBCL Core Genes – DDCG) that are then used in the Denome Prediction Software to uncover the DLBCL subtype. The DDCG Primer Set provided in the kit contains premixed forward and reverse oligonucleotide primers developed for the use of any SYBR Green based qPCR detection, making qPCR reactions compatible with any instrument that detects SYBR Green dye.

Denome prediction algorithm uses the expression of a minimum number of essential genes (Denome Core Genes) to predict expression levels of 8000 genes with over 90% accuracy. Denome qPCR kit comes with a series of gene primers specific to quantify Denome Core Genes, which researchers will use to set up and run reactions in their existing RT-qPCR machines. After which, they can upload the raw data to cloud-based secured servers to be analyzed by Denome prediction algorithm. Results will be delivered to the researcher with expression data of 8000 genes, including publication-quality volcano plots, heatmaps, and gene ontology analysis within 24 hours, unlike an RNA-seq experiment which generally takes 3-4 weeks and also requires specialized technical skills and tedious bioinformatics analysis.